This weeks lab is all about the plasma membrane, its function and its impact on humans through genetic diseases. The data that is necessary to answer the questions for each section is already incorporated into the Skill Check worksheet for that section.
1. The first thing to do is to read the pre-lab which will give you a lot of information about the importance of the plasma membrane in human metabolism and health. At the end of the reading is a link to an online simulation that demonstrates the potential loss or gain of liquid from dialysis bags containing different sugar concentrations when placed for 24 hours in beakers containing a single sugar concentration. Data for this exercise should be recorded on the Skill Check Worksheet, and a graph of the data can be produced in Excel and uploaded along with the exercise.
2. Next, read through the lab itself, paying attention to each part of the lab in the context of the information that follows here.
3. Activity 1 of the lab is designed to demonstrate the movement of small molecules across a semi-permeable membrane. The first thing you should do, is watch the following video that basically walks through the first activity of the lab. You will be able to actually see the results that you will get, and that are described in the Activity 1 section of the skill check worksheet.
Please note that the formula for iodine potassium iodide used in the video (IKI) is different from the usually accepted one that I use in the lab documents and would like for you to use (I2KI)
4. Answer all of the questions on the skill check worksheet for activity 1.
For Activity 2 of the lab exercise, you should
1) read through the directions of the lab,
2) and then look at this video. Although this video does not demonstrate the exact set of experimental conditions as the lab activity, it will give you a good idea of what the cells will look like under isotonic, hypotonic and hypertonic conditions. The view of these cells should give you an idea of what is happening to these cells under each set of conditions.
Activity 3 presents three examples of genetic diseases that are based on mutations that impact membrane structure/function in some way. Read through each of the case studies and then answer the questions that are included in the skill check worksheet for each of them.
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