Mid-Term Lab Practical Review – Group Activity
1. Match each reagent/tool with its associated technique:
a. Antibiotic filter disc
b. Depression slide
e. Inoculating loop & agar plate
g. Malachite green
h. Immersion oil
i. Bibulous paper
j. Hot water bath
_____ Gram stains
_____ Motility testing
_____ Microscopy with the 100X objective
_____ Kirby-Bauer Test
_____ Isolation streaking
_____ Determination of thermal death time
_____ Negative staining
_____ Endospore staining
_____ Drying your prepared slide
2. Outline the steps for preparing a Gram stain, including preparing the smear.
3. You would expect a Mycobacterium species to be: (Gram +/Acid fast). (pick one)
4. Is Brownian motion the same as true motility? Explain.
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